Oxidation, Oxygenation, and Intelligent
Biological Signaling

B) Adaptive Response — Antioxidant Activation

The mild oxidative signal produced by ozone does not only attack pathogens. It also triggers an adaptive response inside the cell.
This activates:

• Glutathione production
• Superoxide dismutase (SOD)
• Catalase(def)
• Nrf2 pathway (master regulator of antioxidant
  defenses)

This process improves redox balance (the balance between oxidation and antioxidant protection).

• As a result, repeated ozone therapy may support:
• cellular resilience
• detoxification pathways
• inflammation balance
• recovery capacity
• resistance to chronic oxidative stress

This adaptive effect is one of the main reasons ozone therapy is used in long-term wellness programs, because the body becomes better at regulating stress instead of only reacting to it.

After the primary reactions are completed, ozone converts back into oxygen (O₂). Ozone therapy can enhance oxygen delivery by: improving red blood cell flexibility increasing 2,3-DPG in red blood cells (master regulator of antioxidant defenses) improving microcirculation supporting oxygen release from hemoglobin

Better oxygen delivery supports:

• ATP production
• cellular repair
• circulation
• metabolic activity
• tissue recovery
• energy production

 

Because oxygen delivery improves after the oxidative signaling phase, this is called the secondary oxygenation effect.

Immediate reaction → signaling molecules

Adaptive response → antioxidant activation

Oxygenation → improved circulation & energy

Ozone (O₃) is a colorless, unstable gas composed of three oxygen atoms.

Because the third oxygen atom is loosely bound, ozone is highly reactive and able to participate in controlled oxidation reactions that can influence biological processes.

For more than a century, medical ozone has been studied and used in different parts of the world for its disinfecting, regulatory, and oxygen-supporting effects.

Ozone therapy does not act like a drug that forces a single effect. Instead, it works by triggering controlled biochemical responses that allow the body to adapt and regulate itself. For this reason, ozone therapy is often used as a supportive or complementary therapy rather than a replacement for conventional treatment.

Ozone therapy has been used in:

• integrative medicine
• biological medicine
• Dentistry
• eye related issues including cataracts *
• wound care
• sports medicine
• rehabilitation
• anti-aging programs
• immune support protocols

 

Medical ozone may influence the body in several different ways depending on the method, dose, and frequency of use.

These actions are often grouped into functional categories.

Adjunctive / Supportive Action

Ozone therapy is often used as a supportive therapy together with other treatments.
It may be used in programs involving:

• chronic illness
• circulation problems
• immune imbalance
• inflammatory conditions
• recovery programs

Ozone does not directly cure these conditions, but it may help support the body's regulatory systems.

---

Blood Oxygenation Support

Ozone therapy may improve the ability of blood to deliver
oxygen.

Possible effects include:

● increased red blood cell flexibility
● improved microcirculation
● increased 2,3-DPG (higher affinity for oxygen which can enhance oxygen delivery)
● better oxygen release into tissues

Improved oxygen delivery supports:

● energy production
● tissue repair
● metabolic activity
● circulation

Medical ozone can be applied in different ways depending on the goal of the therapy.

Each method has its own purpose and level of intensity.

Below are the most commonly used methods.

 

These methods are used for specific areas.

• Ear insufflation
• Nasal / oil bubbler breathing
• Limb bagging
• Topical ozone
• Dental ozone

These methods are often used for skin, joints, infections, or localized circulation problems.

 

Whole-body methods are often used for:

• detox programs
• circulation support
• recovery programs
• wellness maintenance,
• lowering blood pressure
• cellulites

Some ozone procedures must be performed by trained professionals.

• Major autohemotherapy
• Injections
• Prolozone

These should only be done under qualified supervision.

Continue Learning

see also:

• 99 Benefits of Ozone Therapy
• Ozone Around the World
• Ozone Research Library
• Steam Ozone Sauna Therapy
• Ozone Therapy Kits

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* Reference: Healing the Eye the Natural Way
by Edward Kondrot M.D. (Author)

Global Ozone Network – Ozone Around the World

• AAOT – American Academy of Ozone Therapy

• ISCO3 – International Scientific Committee of Ozone Therapy
  Custodians of the Madrid Declaration.

• AEPROMO – Spanish Association of Medical Professionals in Ozone Therapy

• Ozone Research Center (Cuba)
  Founded in 1992, it helped establish clinical protocols now used globally.

• Madrid Declaration on Ozone Therapy
  A global consensus document, translated into 10+ languages and updated every 5 years.

• Key Milestones

  • 1840s – German scientist Christian Friedrich Schönbein discovers ozone (O₃) as a unique form of oxygen.

  • 1873 – Researcher Fox documents ozone’s ability to eliminate microorganisms.

  • 1881 – Dr. Kellogg mentions ozone in treating diphtheria.

  • 1885 – The Florida Medical Association publishes the first medical ozone textbook (Dr. Kenworth).

  • 1893 – First municipal ozone water treatment plant opens in Ousbaden, Netherlands.

  • 1896 – Nikola Tesla patents the first medical ozone generator.

  • 1911 – Dr. Noble Eberhart treats diseases with ozone at Loyola University, Chicago.

  • 1915 – Dr. Wolf uses ozone in German WWI field hospitals for gangrene and wounds.

  • 1932 – Dr. Fish introduces ozone into dentistry and patents the first dental ozone device.

  • 1940s – FDA suppression in the U.S. shifts medicine toward antibiotics.

  • 1970s – Russian doctors treat burns, asthma, and heart conditions with ozone.

  • 1980s – Cuba adopts ozone therapy; the Ozone Research Center is founded in 1992.

  • 2010 – Release of the Madrid Declaration on Ozone Therapy — the first global consensus document.

  • 2020 – The 3rd edition of the Madrid Declaration expands to human, veterinary, and dental medicine.

Find the History of ozone article-link BELOW

Ozone Through Time: A Medical History of Healing with Oxygen

[read more]

• Ozone therapy has a rich, global history, rooted in medical innovation, wartime necessity, and modern science. Below is a detailed historical overview showing how ozone became a cornerstone of integrative medicine — and why it’s still growing in relevance today.
  🌍 1800s–Early 1900s: The Foundations of Ozone Therapy
• 1840 — Ozone (O3) was first discovered by German chemist Christian Friedrich Schönbein.
• 1873 — Fox observed ozone's ability to kill microorganisms, marking the beginning of its disinfection use.
• 1881 — Dr. John H. Kellogg referenced ozone's disinfectant use in diphtheria treatment in his medical texts.
• 1885 — The Florida Medical Association published the first U.S. textbook on ozone therapy by Dr. Charles Kenworthy.
• 1893 — The Netherlands built the world’s first ozone-based water purification plant in Ousbaden. Over 3,000 such plants exist today in the Netherlands alone.
• 1896 — Nikola Tesla patented the first ozone generator and later founded the Tesla Ozone Co. He was the first to ozonate olive oil for therapeutic use.
• 1898 — Drs. Thauerkauf and Luth established the Institute of Oxygen-Ozone Therapy in Germany and began publishing animal studies.
• 🧬 1910–1930s: Early Clinical Use
• 1911 — Dr. Noble Eberhart (Loyola University, Chicago) used ozone for over 10 medical conditions including TB, diabetes, and syphilis.
• 1913 — Dr. Blass founded the first German ozone therapy association.
• 1915 — WWI: Dr. Wolf used ozone topically in German field hospitals to treat gangrene, trench foot, and infected wounds.
• 1932 — Swiss dentist Dr. Fisch published the first ozone-in-dentistry studies and patented the Cytozon ozone device.
• 1935 — Austrian-German surgeon Dr. Edwin Payr demonstrated ozone's wound healing properties.
• 📘 1940s–1960s: From Suppression to Rebirth
• 1940s — The U.S. FDA began seizing ozone generators and promoting pharmaceuticals, pushing ozone into the shadows.
• 1957 — German Dr. Hänsler developed the first modern ozone generator using ozone-resistant plastics.
• 1961 — Dr. Hans Wolff introduced major and minor autohemotherapy with ozone — foundational to modern ozone IV therapies.
• 1970s — Germany created the German Society of Ozone Therapy, helping establish modern research protocols.
• 1970s–1980s — In the Soviet Union, researchers in Estonia and Belarus used ozonated steam for asthma and burns. In 1979, ozonated cardioplegic solution was first used in heart surgery.

📚 Notable Names Who Shaped Modern Ozone Therapy

Professor Manfred von Ardenne (Germany) — Developed the oxygen multi-step therapy (EWOT) and did groundbreaking work combining hyperthermia and ozone sauna treatments, helping bring ozone therapy to mainstream clinics in Europe.

Dr. Robert Rowen (USA) — Often called "the father of medical freedom," Dr. Rowen introduced ozone therapy to West Africa during the Ebola crisis and helped pioneer its use in chronic disease and viral infections.

Ed McCabe (USA) — Author of Flood Your Body with Oxygen, McCabe popularized oxygen-based therapies to the general public and advocated for self-care detox protocols.

Saul Pressman (Canada) — A major contributor to ozone therapy education, Pressman’s writings have trained countless practitioners in safe, home-based ozone use.

🌐 Ozone Associations Worldwide

AAOt – American Academy of Ozonotherapy

ISCO3 – International Scientific Committee of Ozone Therapy (custodians of the Madrid Declaration)

AEPROMO – Spanish Association of Medical Professionals in Ozone Therapy

Ozone Research Center (Cuba) – Founded in 1992, helped establish clinical protocols now used globally

Madrid Declaration on Ozone Therapy – A global consensus document, translated into 10+ languages and updated every 5 years

🌎 1990s–Today: Global Revival

1986 — Cuba officially adopted ozone therapy and soon established the Ozone Research Center in Havana.

2005 — Russia legalized ozone therapy for dermatology and cosmetic use; by 2007 it was approved in obstetrics and neonatology.

2010 — The Madrid Declaration was unanimously approved, becoming the global standard for clinical ozone use.

Today — Ozone therapy is legally practiced in over 60 countries and supported by 50,000+ practitioners globally.

Germany, Russia, Cuba, Spain, and the USA continue to lead in ozone research, clinical protocols, and patient success stories.

🔚 Conclusion: A Time-Tested Therapy Returns to the Spotlight

What began as a humble disinfectant has evolved into a comprehensive therapy for chronic disease, immune support, detoxification, pain, infection, and more.

Ozone therapy is not just a historical curiosity — it’s a rising star in modern functional medicine.

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